http://archives.mycotopia.net/discus/messages/5/28557.html?1103615098 heres some from the vault..,. some grow differnent, temp, growing medium, potency, ect.. where they are found..
The biggest difference is where they were found geographically. There are some physical differences but P. cubensis are all tropical species so for the most part all will grow at the same temp in the same substrate. Also the potency is pretty much to same across the board for Cubes but fluctuates from individual mushroom to mushroom in multispore cultures. You can make this more uniform by cloning a individual fruit and growing out that tissue culture. Variations of Psilocybin and Psilocin Levels with Repeated Flushes (Harvests) of Mature Sporocarps of Psilocybe Cubensis (Earle) Singer. By Jeremy Bigwood and Michael W. Beug Evergreen State College, Olympia, Washington. Summary Analysis of Psilocybe cubensis (Earle) Singer) grown in controlled culture showed that the level of psilocin was generally zero in the first (or sometimes even the second) fruiting of the mushroom from a given culture and that the level reached a maximum by the fourth flush. The level of psilocybin, which was nearly always at least twice the level of psilocin, showed no upward or downward trend as fruiting progressed, but was variable over a factor of four. Samples obtained from outside sources had psilocybin levels varying by over a factor of ten form one collection to the next. Introduction When undertaking quantitative analysis of psilocybin and psilocin levels in the Pacific Northwest species, we generally found large variations from one collection to another even within one species and even when all collections were made from a single location (Beug and Bigwood, 1982). In investigating bio-synthetic pathways in the formation of psilocin and psilocybin in Psilocybe cubensis (Earle) Singer, we also observed variations in psilocybin and psilocin levels from one fruiting to the next (Chilton, 1979). WE therefore stet out to grow a selected Amazonian strain of Psilocybe cubensis (Earle) Singer in carefully controlled cultures and study the variations of psilocybin and psilocin levels with time. We also report here on the observed variation of psilocybin and psilocin levels with repeated flushes from a single culture and the variation observed in other strains. Experimental The strain of Psilocybe cubensis cultivated in this study originated from a spore print taken in the Amazon basin near Pucalpa, Peru (Repke et al., 1977). Mycellia obtained from the spore print was kept as a stock culture on various agars. Since only one flush (fruiting) could be obtained from agar plates, we used a rye-grain medium, described initially in San Antonio (1971), refined by Oss and Oeric (1976), and adapted to "mini-culture" by us. A wide-mouth half-pint jar (~250 ml) was charge with 10 g of rye grain and 15 ml of water and autoclaved. It was then inoculated under sterile conditions with a mycellial culture on agar. Every four days for a period of 28 days, weight per mini-culture. Each flush was harvested as soon as the sporocarps were mature. The mushrooms ere the jars were shaken to distribute the growing mycellia evenly on the grain. In 28 days, the mycellia had covered the grain and the jars were then opened and the grain was cased (covered with a layer about 2 cm deep) with 2 parts peat : 1 part calcium carbonate : 2 parts perlite and/or vermiculite. The mushrooms were "watered" once every two days with 1 ml of sterile water via syringe. The first flush (fruiting) occurred four to five weeks after inoculation (about two weeks after casing). The mini-cultures continued to produce mushrooms for at least 20 weeks provided they remained uncontaminated. They yielded an average of 2.7 g dry weight per mini-culture. Each flush was harvested as soon as the Sporocarps were mature. The mushrooms were immediately freeze-dried, sealed in plastic and stored at—5 degrees Celsius until analysis. Voucher specimens were prepared for deposit in the University of Washington Herbarium (WTU). The extraction procedure and analysis was described in the previous paper. The reversed-phase high performance liquid chromatograms were quantified with a Hewlett-Packard 3380! Reporting integrator-plotter and calibrated against standards from the National Institute on Drug Abuse. We found a linear relationship (plus/minus 10% repeatability) between concentration and peak area from 0.2 to 3 micro-grams total Psilocybin or psilocin. The detection limit was about 0.01 micro-grams psilocybin or psilocin. The HPLC results were qualitatively confirmed by TLC using butanol-acetic acid-water (12:3:5). Results We found that the levels of psilocybin varied somewhat unpredictably from one flush to the next, but generally were much the same on the last flush as they were on the first flush (Table 1). Psilocin, on the other hand, generally was absent in the first one or two flushes, each maximized by the fourth flush, and then appeared to start to decline (Table 1). Unfortunately, we could generally not follow the decline appreciably since five flushes is normally the maximum we can get before the mycellia stops fruiting. (With mini-culture 1, we obtained a sixth flush but the fifth flush was totally consumed in another experiment and is not reported here.) In two other strains grown by other sources, we also observed nearly complete absence of psilocin in the first flush . In These, we analyzed the caps and stems separately and found that the caps generally contained twice as much psilocybin as the stems, but that the small amount of psilocin present was entirely in the stems (Table 2). In contrast, our Amazon strain Had a trace of Psilocin in the cap but not in the stem. The cap and stem contained equal amounts of psilocybin. Finally, we analyzed five street samples of Psilocybe cubensis for which we did not know the flush number or the precise growing conditions (Table 3). We found highly variable levels of psilocybin and low levels of psilocin. TABLE 1 The dry weight variation of psilocybin and psilocin levels in Psilocybe cubensis as a function of flush number (quantified by HPLC. Mini-culture No. 1 Psilocybin—Psilocin (mg/g) (mg/g) 1 8.3---------------0.5 2 6.5---------------1.5 3 13.3---------------1.0 4 4.8---------------2.6 5 --/--------------/-- 6 6.8---------------0.5 Mini-culture No. 2 Psilocybin—Psilocin (mg/g) (mg/g) 1 5.1---------------0 2 7.3---------------0 3 4.7---------------1.7 4 3.7---------------2.9 5 5.2---------------2.2 6 --/--------------/-- Mini-culture No. 3 Psilocybin—Psilocin (mg/g) (mg/g) 1 7.6---------------0 2 6.2---------------0 3 5.3---------------0.9 4 3.2---------------1.8 5 6.7---------------1.7 6 --/--------------/-- TABLE 2 Distribution of psilocybin and psilocin in the cap versus the stem in three strains of Psilocybe cubensis cultivated on rye-grain substrate M. R. strain- First flush Psilocybin Psilocin (mg/g) (mg/g) Caps 9.7---------0 Stems 4.2---------0.35 Ecuadorian Strain First flush Psilocybin Psilocin (mg/g) (mg/g) Caps 7.6--------0 Stems 4.7--------0.4 Amazon Strain First flush Psilocybin Psilocin (mg/g) (mg/g) Caps 5.7--------0.1 Stems 5.7--------0 TABLE 3 Psilocybin and psilocin levels in dried psilocybe cubensis "street samples" (all samples were from material cultivated on a rye-grain substrate) Sample Psilocybin Psilocin No. (mg/g) (mg/g) 1---------------5.6----------------0 2---------------6.2----------------0 3---------------0.7----------------0.3 4---------------0.7----------------0.3 5---------------1.3----------------0.3 Conclusions We found that the level of psilocybin and psilocin varies over a factor of four among various cultures of Psilocybe cubensis grown under rigidly controlled conditions, while specimens from outside sources varied tenfold. IT is clear that entheogenic (Ruck et al., 1979) and recreational users of this species have no way of predicting the amount of psilocybin and psilocin that they are ingesting with a given dry weight of the mushrooms. It thus seems likely that variations in the subjective experience will not only come from the effects of set and setting but will also stem in very real measure rom large dosage differences. References Beug, Michael W. and Jeremy Bigwood. 1982. Psilocybin and psilocin levels in twenty species from seven genera of wild mushrooms in the Pacific Northwest, U.S.A. Journal of Ethnopharmacology vol. 5:271-285. Chilton, Scott., Bigwood, Jeremy and R. E. Jensen. 1979. Psilocin, Bufotonine and serotonin : Historical and Bio-synthetic Observations. Journal of Psychedelic Drugs Vol. 11:61-69. Oss, O. T. and Oeric, O. N. 1976. Psilocybin Magic Mushroom Growers Guide. And/Or Press, Berkeley, California. Repke, Carl A. P., Leslie, Dale T., and Gastón Guzmán. 1977. Psilocybe, Conocybe and Panaeolus. Lloydia Vol. 40:566-578. Ruck,Carl A. P., Bigwood, Jeremy., Staples, Danny., Ott, Jonathan and R. Gordon Wasson. 1979. Entheogens. Journal of Psychedelic Drugs Vol. 11:145-147. San Antonio, J. P. 1971. A laboratory method to obtain fruit from cased grain spawn of the cultivated mushroom : Agaricus bisporus. Mycologia vol. 63:16-21.
Basically when it comes to growing them there will be no difference if you are doing it indoors. A cube is a cube.
