I can’t figure out how to PM someone or set my signature but hey I’m writing a long in depth tek for this community. On how to properly grow a large amount of mushies easily.
I do provide spore prints and syringes to several large spore companies. I may be able to provide spores to some people if they have interest
I’m hoping this will be a complete and comprehensive guide on how to grow 3/4 of a pound of Psilocybe Cubensis mushrooms in under 3 months start to finish. This will also be a fairly involved crash course on growing Magic Mushrooms. I see some old outdated information on Cubensis mushrooms and thought I would help this community out with some newer methods.
1. Construction of a glove box
3. Source of inoculation
A: Spore syringe from print
B: Liquid Culture (LC)
4. Spawn choices
A: Grain Prep (rye and WBS)
5. How to prepare a Monotub and pasteurize your coir substrate (This is still my tek but it was written out for another forum. I just edited to fit with the other information I am providing.)
1. A Still Air Box or Glove Box (SAB/GB) is a mycologist’s best friend. You are going to curse trichoderma and other sorts of contams if you do this long enough. One safeguard we use to have nice clean healthy jars is the use of a GB (Glove box). There are millions of particles of nastiness all thru out the air just waiting to contaminate your jars and make them all sorts of funky colours. The principal behind a GB is that there is no moving air in in and all the mold and bacteria particles have settled on the ground. You are now working in an area that is virtually contam free. A GB is very easy to construct. Go out and get a rubbermaid tote a clear one is better so you can see thru but you can use a coloured one and cut a hole in the top and cover with saran wrap. Cut 2 holes in the sides of your GB big enough that you can stick your hand thru and that is pretty much it. To prepare your GB for use spray it with soapy water, 1:10 bleach:water or lysol. Wait 5 minutes and goto work. If using lysol wait at least 10 minutes. You dont want to spark your lighter and blow yourself up.
As you can see my GB is pretty ghetto, but it works and I wouldn’t work without it
2. To construct lids you will need
mason jar lids
1/4 inch and 3/8 inch drill bits
RTV high temp silicone gasket silicone or any silicone rated above 280F
Tyvek you can get it from USPS envelopes or you can buy construction tyvek but make sure there are no holes in the construction tyvek
Cut a ¼ inch hole in between the middle of the jar and the lip of it. Drill the 3/8 hole across from it. Take RTV and use it to glue a tyvek quarter sized piece over the 3/8 inch hole. Use the RTV to fill in the ¼ inch hole with globs sticking out both sides of it. Your lids should look like this.
Except for one thing I used tyvek for both sides. Since these lids pictured here I only tyvek one side. The other material on lids pictured here is synthetic filter material designed for mycology. Tyvek works fine and is free for americans at their post office.
3. Now to inoculate your cakes or jars you are going to need a source of inoculation. I am going to go into 2 different methods of inoculation. No matter what you are going to need a spore print or a syringe.
How to make a syringe
Items you will need inside your GB
Shot glass(Make sure you clean it out well with bleach and water)
Scalpel or knife something metal to scrape the spores off of the print
Syringe filled with clean sterile water
Put a pot of water to boil on the stove. Once boiling suck water into the syringe and expel it into the sink. Do this 5-6 times and leave the water in the syringe to cool. You now have sterile water in a syringe. Place all items into your GB. First open your print so that you will be able to open it and get at the spores. Flame sterilize your scraping tool. Hold the tip of the lighter flame to your metal instrument until it is glowing red hot. Re sterilize your shot glass with an alcohol wipe. Scrape a small portion of spores into your shot glass. The less spores in your syringe is better. Less genetic competition. Heat the needle tip red hot and slowly expel the water in your syringe into your shotglass with spores. Be careful when you first depress the plunger. I’ve pushed too hard and water and spores goes everywhere. Stir the mix up with the needle tip and suck it back into your syringe. Let your syringe sit for 24 hours to rehydrate the spores.
How to make a Liquid Culture(LC)
A LC(Liquid Culture) is a great way to have a lot of inoculate ready. With the added benefit of growth showing within 24 hours.
Items needed for an LC
500 or 600 ML of water
Source of sugar
A: Karo 1 tblspoon
B: Extra Light Malt Extract or Light Malt Extract (ELME/LME) 1 gram
C: Turbinado sugar also referred to as Sugar in the Raw (SITR) Free packets of 5g from starbucks 10 grams
Lid as built above
Piece of glass or marble
If using karo or SITR use 500 ML of water if using ELME use 600 ml of water. Pour water into the mason jars. You may make 2 if not using quart mason jars. Add marble or piece of glass. This will help break apart the mycelium as in grows in the LC. PC the LC for 20 min at 15 PSI. Inoculate with 1 ml of spore solution from a spore syringe. Let this grow until nice and thick with white mycelium Just because an LC looks clean. Doesn’t mean it is. Make sure to shoot one test jar to test the LC. Making sure it grows nice and healthy without contams. Once you have a clean tested LC you can store this for years in your fridge
Nice thick SITR LC.
So you have the choice of using the LC or 1 ML of Multi Spore(MS) solution from your syringe for every jar. There are several other methods of inoculation but I am just going to stick with these two forms for now. I can give a crash course on agar, GLC or G2G if the need arises.
4. Spawn Choices
A: Grain Prep
This is the same for Rye or Wild Bird Seed(WBS) with one exception for the boil. Rye I simmer for 20 minutes. Wbs I just bring to a boil and turn off. Measure out your grain. Either rye or WBS I try 2 cups per quart. Depending on how much moisture is present it will swell to different volumes. Just A good starting point is 2 cups per quart. Pour the dry grain into a bucket and fill 1/3 over with boiling water. Let this sit for 1.5 hours or more and it will swell up. Strain out the water and put into a large pot and cover the grain with water and some. Bring to a boil. If you are doing WBS turn off the stove and remove from the element once it reaches a boil. If doing rye turn the heat down and simmer for 20 minutes. Strain the hot grain into a colander and let sit for an hour but mixing it up every 5-10 minutes. Load your jars with your prepared grain 2/3 rds full. Cover your lids with aluminum foil and Pressure Cook(PC) for 1.5 hours at 15 PSI
Edited by UnnamedGrower, September 24 2011 - 11:08 PM.